Anti TDP-43, phospho Ser410

Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho Ser410 pAb was prepared from rabbits immunized with phospho-peptide [CMDSKSS(p)GWGM]. This pAb recognizes human TDP-43 phosphorylated on serine 410 and is validated for western blot, ELISA and IHC(f) analyses of TDP-43 proteinopathy.

TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.

Specifications

• Product type                primary antibody
• Immunogen                 CMDSKSS(p)GWGM, S(p): phosphoserine 410
• Raised in                     rabbit
• Source                         serum
• Form                           liquid antiserum with 0.05% NaN3 as a preservative
• Volume                       100 uL
• Label                           unlabeled
• Specificity                   phospho-TDP-43
• Cross reactivity           human
• Storage                        below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.

Recommended dilutions

• Western blotting: 1/1000-1/3000
• Immunohistochemistry: 1/1000-1/5000
• ELISA: 1/1000-1/5000
• Other applications not tested
• Optimal dilutions/concentrations should be determined by end user

References

1. Hasegawa M, Arai T, Nonaka T, et al. Phosphorylated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Ann Neurol. 2008; 64(1):60–70. doi:10.1002/ana.21425