To deliver antibodies into living cell. It can easily introduce antibodies into living cell effcientlly by usuing function of artificial cell penetrating peptide, controlling cytotoxicity.
Features
- Suitable for use with 10% serum-containing media.
- Suitable for various antibodies(polyclonal, monoclonal). It is also possible to introduce fluorescently labeled antibodies and enzyme-labeled antibodies.
- Stable at 4 ° C for 6 months. The activity does not decrease even after freezing and thawing (10 times).
- Cell lines with a proven track record (will be added at any time)
- Ready-to-use. Easy and quick procedure.
- Very low cytotoxicity.
Specificity: Endotxin level < 0.05 EU / ul
Antibody transfectionactivity (relative value)* 80 - 150 %
* (BETA) -galactosidase-conjugated goat IgG was transfected to HeLa cells and incubated for 4 hrs. Antibody transfection activity was estimated by (BETA)- galactosidase activity in lysates of the cells. The values represent relative activity (%) to that of Lot N12J15 as standard.
References
1) FEBS Journal (2015) 282: 142-152.
Effective capture of proteins inside living cells by antibodies indirectly linked to a novel cell-penetrating polymer-modified protein A derivative.
Shoko Itakura, Susumu Hama, Hisafumi Ikeda, Naoto Mitsuhashi, Eiji Majima and Kentaro Kogure.
2) Mol Cell Biochem (2015) 404: 25-30.
Effects of employment of distinct strategies to capture antibody on antibody delivery into cultured cells.
Kana Kuwahara, Kazuki Harada, Ryohei Yamagoshi, Takenori Yamamoto, Yasuo Shinohara.