Recombinant Hepatitis B Surface Antigen (adw)

Suitable for use in ELISA and Western blot. Each laboratory, should determine an optimum working titer for use in its particular, application. Other applications have not been tested but use in such assays, should not necessarily be excluded.

Hepatitis B Virus Surface Antigen

Hepatitis, B Virus (HBV) infection induces a disease state characterised by liver, damage, inflammation and viral persistence. Infection also increases the risk, of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of, viruses. Its genome consists of partially double stranded circular DNA. The, DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is, surrounded by a spherical envelope (surface antigen or HBsAg). The core, antigen shares its sequences with the e antigen (HBeAg) but no cross, reactivity between the two proteins has been observed. The HBV genome also, encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis, B infection is normally diagnosed from serological tests that detect HBsAg, but as the disease progresses this antigen may no longer be present in the, blood and tests for HBcAg are used. If HBsAg can be detected in the blood for, longer than six months, chronic hepatitis B is diagnosed. The antigenic, determinant of the protein moiety of the HBsAg determines specific, characteristics of different serotypes and provides the basis of, immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs, of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

HBsAg (adw)

20mM Na2HPO4 and, 30mM Sodium chloride, pH 7.4

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