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Anti-tubulin polyAb pan specific (sheep) (Trial size)

Anti-tubulin polyAb pan specific (sheep) (Trial size)

Item no.	ATN02-S
Manufacturer	Cytoskeleton
Amount	1 x 25 ul
Quantity options	2 x 100 ul 1 x 100 ug 3 x 100 ug 1 x 25 ul
Category	Antibodies Cell Biology Cytoskeleton Primary Antibodies Mikrotubules
Type	Antibody
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Weight (grams)	16
Product Uses	This antibody is recommended for detection of tubulin in all yeast, fungi, and animal species (Fig. 2). The following protocols have been tested with this antibody:
Material	The anti-tubulin antibody (Cat. # ATN02) is an affinity purified sheep polyclonal antibody that reacts to alpha and beta tubulin. The immunogen used for antibody production was a mixture of purified tubulins and therefore the antibody has broad species cross reactivity from yeast to humans. Porcine extract (Cat. # EXT03) is included as a positive control and ATN02 identifies a characteristic tubulin band at 55 kD on Western blots (see Fig. 1). ATN02?is supplied as a lyophilized white powder.
RRID	AB_10708807
Figure 1 Legend	Western blot analysis of anti-tubulin antibody.Protein samples were separated by electrophoresis and transferred to PVDF membrane as described in the methods. ATN02 antibody was diluted to 500 ng/ml (1:1000) for Western blot analysis. Tubulin was detected in 10 ug of bovine brain extract (see arrow at 55 kD). Molecular weight markers are from Invitrogen.
Figure 2 Legend	Figure 2. Western blot of cell extracts probed with anti-tubulin polyclonal antibody (Cat. # ATN02). Chemiluminescence detection of tubulin in extracts of Drosophila S2 cells (50 ug, lane 1) Xenopus A6 cells (50 ug, lane 2), mouse Swiss 3T3 cells (50 ug, lane 3), rat NRK cells (50 ug, lane 4), human HeLa cells (50 ug, lane 5), and bovine brain (50 ug, lane 6) at 55 kD (see arrow). The blot was probed with a 500 ng/ml (1:1000) dilution of ATN02
Figure 3 Legend	Figure 3. Immunofluorescence image of mouse Swiss 3T3 cells stained with Anti-Tubulin polyclonal antibody (Cat. # ATN02). Swiss 3T3 cells were grown to semi-confluency and fixed with methanol. Immunofluorescence staining using 2.5 ug/ml (1:200 dilution) ATN02 antibody is shown (red). The primary antibody was detected with a 1:500 dilution of anti-sheep rhodamine conjugated antibody. DNA (blue) was stained with 100 nM DAPI in PBS. Photograph was taken with a 100X objective lens.
Figure 4 Legend	Figure 4. Immunofluorescence image of Arabidopsis thaliana metaphase cell stained with Anti-Tubulin polyclonal antibody (Cat. # ATN02).Microtubules are pseudocolored in green and chrmosomes in red. Image courtesy of Dr. Bo Liu, Department of Plant Biology, University of California, Davis.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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