Tau (bovine brain)

Question 1: Which tau epitopes are phosphorylated?

Answer 1: Tau is a phosphoprotein with 79 potential serine and threonine phosphorylation sites on the longest tau isoform. Phosphorylation has been reported on approximately 30 of these sites in normal tau proteins. The tau protein we provide is from bovine brain (Cat. #TA01) and Coomassie Blue staining demonstrates multiple tau isoforms exist in the purified bovine tau, some of which are likely to be phosphorylated isoforms. The exact epitopes that are phosphorylated will need to be determined empirically.

Question 2: Will this bovine tau modify polymerization of tubulin from porcine?

Answer 2: Yes, the bovine tau (Cat. #TA01) will bind to porcine tubulin due to the high sequence homology of tau and tubulin between mammalian species. Upon binding to tubulin, polymerization is enhanced.