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Question 1: After reconstituting the lyophilized protein with water, what is the composition of the buffer the cofilin protein is in?
Answer 1: The protein should be reconstituted to 5 mg/ml by the addition of 20 µ, l of distilled water. The protein will be in the following buffer, 10 mM Tris pH 8.0, 1 mM EGTA pH 8.0, 5% sucrose, and 1% dextran. In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration
Question 2: To separate the F-actin bound to cofilin, is it absolutely necessary to spin the sample at 100, 000 x g?
Answer 2: If possible we recommend spinning the F-actin/cofilin complex at 100, 000 x g to absolutely insure that the protein complex pellets. Check with your university&rsquo, s core facilities or neighboring labs for access to an ultracentrifuge. However, in a pinch, spinning at 50, 000 x g should pellet the majority of the complex.
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
CF01-A.pdf
CF01-A-datasheet.pdf
CF01-A-safety-datasheet.pdf
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