Laminin HiLyte488™ (mouse tumor)

AT

1-2 Weeks

14

Laminin-1 is purified from EHS tumor tissue and is free of the laminin binding protein entactin which is a common contaminant in some laminin preparations (150 kDa). Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. ?The laminin is >90% pure (Figure 1).
The protein is modified to contain covalently linked HiLyte 488^TMdyes (3) at random surface lysines. An activated ester of HiLyte 488^TMis used to label the protein. Labeling stoichiometry is determined by spectroscopic measurement of protein and dye concentrations. Final labeling stoichiometry is 2-5 dyes per protein molecule (Figure 2). The material is guaranteed to contain <15% of free dye and >85% of dye conjugated to laminin. HiLyte 488^TMlaminin can be detected using a filter set of 502nm excitation and 527nm emission.
Laminin runs as individual subunits on SDS-PAGE with an apparent molecular weight of 400 and 225 kDa (Figure 1). LMN02 is supplied as an off white lyophilized powder. Each vial of LMN02 contains 20 ug protein.