« Back
Home /
MAP Fraction

MAP Fraction

Item no.	MAPF-C
Manufacturer	Cytoskeleton
Amount	5 x 100 ug
Quantity options	1 x 100 ug 5 x 100 ug
Category	Oncology Neuroscience Neural Lineage Markers Targets for Rodent Neurosciences Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton Mikrotubules By Organ Testicular & Prostate Cancer Glial Cell Markers
Type	Proteins
Specific against	other
Purity	Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel. MAP2 constitutes 70% of the total protein (see Figure 1).
Citations	Cho, H. P., Liu, Y., Gomez, M., Dunlap, J., Tyers, M. and Wang, Y. (2005). The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules. Mol. Cell. Biol. 25, 4541-4551. Satish, L., Blair, H. C., Glading, A. and Wells, A. (2005). Interferon-inducible protein 9 (CXCL11)-induced cell motility in keratinocytes requires calcium flux-dependent activation of &mu,-calpain. Mol. Cell. Biol. 25, 1922-1941. Teckchandani, A. M., Birukova, A. A., Tar, K., Verin, A. D. and Tsygankov, A. Y. (2005). The multidomain protooncogenic protein c-Cbl binds to tubulin and stabilizes microtubules. Exp. Cell Res. 306, 114-127. Mamoune, A., Luo, J. H., Lauffenburger, D. A. and Wells, A. (2003). Calpain-2 as a target for limiting prostate cancer invasion. Cancer Res. 63, 4632-4640. Nielsen, F. C., Nielsen, J., Kristensen, M. A., Koch, G. and Christiansen, J. (2002). Cytoplasmic trafficking of IGF-II mRNA-binding protein by conserved KH domains. J. Cell Sci. 115, 2087-2097. Vallee, R. B. 1982. J. Cell Biol. 92, 435-442 Kuznetsov S. A., et al. 1981. FEBS Lett. 135, 241-244
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	tubulin, MAP, microtubule associated protein, bovine MAP, MAP fraction
Similar products	MAP, tubulin, microtubule associated protein, bovine MAP, MAP fraction
Shipping Condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: What proteins comprise the microtubule-associated protein fraction? Answer 1: The MAPF is comprised of a mixture of MAP1, MAP2 and tau proteins. Question 2: Has the MAPF product been shown to significantly affect tubulin polymerization? Answer 2: Yes, the biological activity of the MAPF can be determined by the ability of 0.1 mg/ml MAPF to enhance the polymerization rate (Vmax) of purified bovine brain tubulin in vitro. Stringent quality control ensures that the MAPF proteins will stimulate tubulin polymerization approximately five fold when compared to tubulin polymerization without MAPF proteins.
Weight (grams)	32
Product Uses	Positive control for the study of microtubule binding proteins Investigation of the the effect of MAPs on microtubule dynamics Substrate for many protein kinases
Material	A microtubule associated protein (MAP) fraction has been isolated from porcine brain by temperature induced tubulin polymerization followed by ionic exchange chromatography over a phosphocellulose matrix and salt elution (1, 2). The MAP fraction protein is supplied as a white lyophilized powder
Biological Activity	The biological activity of the MAP fraction is determined by the ability of 0.1 mg/ml MAP fraction to enhance the polymerization rate (Vmax) of purified bovine brain tubulin (Cat. # TL238) in vitro. Stringent quality control ensures that the MAP fraction protein will stimulate tubulin polymerization at least five foldWhen compared to tubulin polymerization without MAP fraction.
Figure 1 Legend	Figure 1. Microtubule associated protein fraction purity determination. A 10 ug sample of MAPF was separated by electrophoresis in a 12% SDS-PAGE system, and stained with Coomassie Blue.
Figure 2 Legend	Figure 2: Tubulin polymerization in the presence and absence of microtubule associated protein fraction. Tubulin polymerization reactions were carried out as in BK006 with 5% glycerol containing 2 mg/ml of pure bovine brain tubulin (Cat. # TL238 ) being polymerized in the presence and absence of 0.1 mg/ml MAP fraction protein.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

MAPF-C.pdf

MAPF-C-datasheet.pdf

MAPF-C-safety-datasheet.pdf