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Question 1: If the pre-formed microtubules are resuspended, aliquoted and snap-frozen as directed, will they depolymerize and need to be re-polymerized or is the polymerized state maintained during one freeze and thaw cycle?
Answer 1: The pre-formed microtubules ((Cat. #MT002) will not depolymerize upon one freeze thaw cycle. Thaw rapidly in a room temperature water bath and then keep at room temperature until ready to use. However, when aliquoting for snap-freezing, pipette smaller volumes (10-20 ul) since the concentration of the microtubules will be 1 mg/ml (based on datasheet instructions).
Question 2: What is the difference between the two different versions of pre-formed microtubules (Cat. #MT001 and MT002)?
Answer 2: The only difference between the MT001 pre-formed microtubules and the MT002 pre-formed microtubules is the source of the tubulin used to product the microtubules. MT001 uses bovine brain and MT002 uses porcine brain. In side-by-side comparisons (please see the MT002 datasheet), the microtubule-activated kinesn ATPase activity (Cat. #BK053) of MT001 (bovine) and MT002 (porcine) microtubules was compared. Both sources of microtubules produced the same results with this kinesin ATPase end-point assay (Cat. #BK053).
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
MT002-A.pdf
MT002-A-datasheet.pdf
MT002-A-safety-datasheet.pdf
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