Comparison

Human 5-LO (Arachidonate 5-Lipoxygenase) CLIA Kit

Item no. ELS-E-CL-H0197
Manufacturer Elabscience
Amount 96 T
Quantity options 24 T 48 T 96 T 96 T
Category
Type Clia
Applications CLIA
Specific against Human (Homo sapiens)
Sensitivity 37.5 pg/mL
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Alias ALOX5, 5-LOX, LOX5, 5LPG, LOG5,
Shipping Condition Cool pack
Hazard information Comply with cargo safety certificate
Available
Manufacturer - Type
Sandwich-ELISA
Manufacturer - Category
CLIA Kits
Manufacturer - Targets
5-LO
Shipping Temperature
Ice bag
Storage Conditions
2-8℃/-20℃, 6 months
Description
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 5-LO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 5-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human 5-LO. You can calculate the concentration of Human 5-LO in the samples by comparing the RLU value of the samples to the standard curve.
Abbreviation
5-LO
Assay time
3 h 30 min
Detection Type
Chemiluminescence
Tested Sample Types
Tissue homogenates, cell lysates and other biological fluids
Test Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 5-LO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 5-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human 5-LO. You can calculate the concentration of Human 5-LO in the samples by comparing the RLU value of the samples to the standard curve.
Usage
This ELISA kit applies to the in vitro quantitative determination of Human 5-LO concentrations in Tissue homogenates, cell lysates and other biological fluids.
Recovery Rate
80%-120%
Precision
Both intra-CV and inter-CV are < 10%.
Category ID III
CLIA Kits

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 T
Available: In stock
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