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Nitroimidazole (metronidazole) ELISA Kit

Nitroimidazole (metronidazole) ELISA Kit

Item no.	ABE-AE81034SF-5x96T
Manufacturer	Abebio
Amount	5x96T
Category	Food Safety Antibiotics ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	other
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Abbreviation	metronidazole
Range	0.05-12.8 ppb
Sensitivity	0.05 ppb
Sample Type	Fish, shrimp, poultry, liver, honey, etc.
Detection Method	Competitive ELISA
Analysis Method	Quantitive
Assay Duration	1-3h
Sample Volume	10-200 uL
Detection Wavelengt	450 nm
Availability	3-5 working days
Test principle	This assay is based on the competitive enzyme immunoassay for the detection of metronidazole in the sample. The coupling antigens are pre-coated on the micro-well stripes. The metronidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-metronidazole antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the metronidazole in it. This value is compared to the standard curve and the metronidazole concentration is subsequently obtained.
Product Overview	5-Nitroimidazole is an imidazole derivative that contains a nitro group. Several derivatives of nitroimidazole constitute the class of nitroimidazole antibiotics that have been used to combat anaerobic bacterial and parasitic infections. Perhaps the most common example is metronidazole (Flagyl). Other heterocycles such as nitrothiazoles (thiazole) are also used for this purpose. Nitroheterocycles may be reductively activated in hypoxic cells, and then undergo redox recycling or decompose to toxic products.
Precision	Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
Stability	The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).
Storage	Store at 2-8C. Please refer to Instruction Manual.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5x96T

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Delivery expected until 9/25/2025

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