Comparison

Recombinant human Isocitrate Dehydrogenase 1/IDH1 protein

Item no. NKM-ATGP1284-20ug
Manufacturer NKMAX
Amount 20 ug
Quantity options 100 ug 20 ug 500 ug
Category
Type Proteins Recombinant
Format Liquid
Applications SDS-PAGE
Specific against Human (Homo sapiens)
Host E.coli
Conjugate/Tag HIS
Purity > 95% by SDS-PAGE
Sequence <MGSSHHHHHH SSGLVPRGSH> MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS
Citations Thorsness P.E. et al. (1987) J. Biol. Chem. 262: 10422-10425.
Geisbrecht B.V. et al. (1999) J. Biol. Chem. 274: 30527-30533.
NCBI NP_005887.2
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Isocitrate dehydrogenase [NADP] cytoplasmic,IDCD,IDH,IDP,IDPC,PICD
Similar products IDH1, IDCD, IDH, IDP, IDPC, PICD, Isocitrate dehydrogenase [NADP] cytoplasmic, Isocitrate Dehydrogenase1, ATGP1284-10ug, ATGP1284-20ug, ATGP1284-50ug, ATGP1284-100ug, ATGP1284-250ug, ATGP1284-500ug, ATGP1284-1mg, ATGP1284-10, ATGP1284-20, ATGP1284-50, ATGP1284-100, ATGP1284-250, ATGP1284-500, ATGP1284-1
Available
Manufacturer - Type
Recombinant Protein
Manufacturer - Applications
SDS-PAGE, Enzyme Activity
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
48.8 kDa (434aa) confirmed by MALDI-TOF
Description
IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Antigen Species
Human
Gene Category
Cancer
Domain
1-414aa
Bioactivity Comment Only
Specific activity is > 18, 000pmol/min/ug. One unit will oxidize 1.0pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.
Species of Protein
Human
Manufacturer - Search Terms
Isocitrate Dehydrogenase1, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD, ATGP1284-10ug, ATGP1284-20ug, ATGP1284-50ug, ATGP1284-100ug, ATGP1284-250ug, ATGP1284-500ug, ATGP1284-1mg, ATGP1284-10, ATGP1284-20, ATGP1284-50, ATGP1284-100, ATGP1284-250, ATGP1284-500, ATGP1284-1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
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