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Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Item no.	BOS-EZ0413
Manufacturer	Boster
Amount	5 plates/kit
Quantity options	5 plates/kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Carcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<1 pg/ml
Sequence	Belongs to the intercrine alpha (chemokine CxC) family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Interleukin-8,IL-8,C-X-C motif chemokine 8,Chemokine (C-X-C motif) ligand 8,Emoctakin,Granulocyte chemotactic protein 1,GCP-1,Monocyte-derived neutrophil chemotactic factor,MDNCF,Monocyte-derived neutrophil-activating peptide,MONAP,Neutrophil-activating protein 1,NAP-1,Protein 3-10C,T-cell chemotactic factor,MDNCF-a,GCP/IL-8 protein IV,IL8/NAP1 form I,Interleukin-8,(Ala-IL-8)77,GCP/IL-8 protein II,IL-8 (1-77),IL8/NAP1 form II,MDNCF-b,IL-8 (5-77),IL-8 (6-77),(Ser-IL-8)72,GCP/IL-8 protein I,IL8/NAP1 form III,Lymphocyte-derived neutrophil-activating factor,LYNAP,MDNCF-c,Neutrophil-activating factor,NAF,IL-8 (7-77),GCP/IL-8 protein V,IL8/NAP1 form IV,IL-8 (8-77),GCP/IL-8 protein VI,IL8/NAP1 form V,IL-8 (9-77),GCP/IL-8 protein III,IL8/NAP1 form VI,CXCL8,IL8
Available
Manufacturer - Category	EZ-Set™ ELISA Kits (Ab Pairs)
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	11098 MW
Gene Name	CXCL8
Gene Full Name	Interleukin-8
Manufacturer - Specificity	Natural and recombinant human IL-8
Background	Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: S28-S99
Protein Name	Interleukin-8
Protein Function	IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively.
Subcellular Localization	Secreted.
Description	Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Sequence Similarities	Belongs to the intercrine alpha (chemokine CxC) family.
Sample Data	Concentration (pg/ml) \| 0 \| 15.6 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 O.D. \| 0.000 \| 0.082 \| 0.149 \| 0.281 \| 0.556 \| 0.936 \| 1.594 \| 2.071
Kit Components	Catalog number \| Description \| Quantity \| Storage of opened/reconstituted material EZ0413-CA \| Mouse anti- human CXCL8 monoclonal antibody (Capture Antibody) \| 500 µ l, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0413-DA \| Biotinylated goat anti- human CXCL8 polyclonal antibody (Detection Antibody) \| 500 µ l, 5.9 µ g/mL (recommended dilution 1:100) AR1103 \| Avidin-Biotin-Peroxidase Complex (ABC) \| 500 µ l (recommended dilution 1:100) EK0413-ST \| Lyophilized recombinant human CXCL8 standard \| 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.
Anticoagulant	heparin, EDTA or citrate
Material Required But Not Provided	1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T). Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Manufacturer - Alias	Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil chemotactic factor, MDNCF, Monocyte-derived neutrophil-activating peptide, MONAP, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, T-cell chemotactic factor, MDNCF-a, GCP/IL-8 protein IV, IL8/NAP1 form I, Interleukin-8, (Ala-IL-8)77, GCP/IL-8 protein II, IL-8 (1-77), IL8/NAP1 form II, MDNCF-b, IL-8 (5-77), IL-8 (6-77), (Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF-c, Neutrophil-activating factor, NAF, IL-8 (7-77), GCP/IL-8 protein V, IL8/NAP1 form IV, IL-8 (8-77), GCP/IL-8 protein VI, IL8/NAP1 form V, IL-8 (9-77), GCP/IL-8 protein III, IL8/NAP1 form VI, CXCL8, IL8
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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BOS-EZ0413-datasheet.pdf

BOS-EZ0413-safety-datasheet.pdf