Human IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

IL12A

Natural and recombinant human IL-12(p70)

Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)

Human IL-12 (p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL-12 (p70) in cell culture supernatants and serum.

Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500
O.D. | 0.019 | 0.069 | 0.126 | 0.239 | 0.441 | 0.834 | 1.441 | 2.086

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

There is no detectable cross-reactivity with other relevant proteins.