Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit

Quick ELISA Kits

APOA1

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APOA1/Apolipoprotein A-I in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200
O.D. | 0.021 | 0.157 | 0.271 | 0.483 | 0.814 | 1.311 | 1.870 | 2.285

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 8188 | 7998 | 8004 | 7332 | 7880 | 330.96 | 4.2%
Sample 2 | 19829 | 19750 | 20658 | 19659 | 19974 | 619.19 | 3.1%
Sample 3 | 93784 | 86245 | 87457 | 88574 | 89015 | 4005.67 | 4.5%
*number of samples for each test n=16.

Description|Quantity|Volume
Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
Standard||2
Antibody A|1|3ml
Antibody B|1|3ml
Sample Diluent|1|15ml
TBS-T Wash Buffer (25x)|1|12ml
Color Developing Reagent (TMB)|1|10ml
Stop Solution|1|10ml
Adhesive Plate Sealers|2|Piece

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.