Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit

Sandwich-ELISA

PAI-1

2-8℃/-20℃, 6 months

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PAI-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PAI-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PAI-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PAI-1. You can calculate the concentration of Rat PAI-1 in the samples by comparing the RLU value of the samples to the standard curve.

3 h 30 min

Serum, plasma and other biological fluids

This ELISA kit applies to the in vitro quantitative determination of Rat PAI-1 concentrations in Serum, plasma and other biological fluids.

Both intra-CV and inter-CV are < 10%.