ESM1/Endocan ELISA Kit (Mouse)

ELISA Kit

Wet Ice

endothelial cell-specific molecule 1

Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

NP_076101.1

Expression system for standard: NS0; Immunogen sequence: W20-R184

Assay Methodology: Quantitative Sandwich Immunoassay

Aviva's mouse ESM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ESM1 has been precoated onto 96-well plates. Standards(NS0, W20-R184) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse ESM1 amount of sample captured in plate.