Chemerin/RARRES2 ELISA Kit (Mouse)

ELISA Kit

Wet Ice

retinoic acid receptor responder (tazarotene induced) 2

Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

NP_082128.1

Expression system for standard: E.coli; Immunogen sequence: T20-K162

Assay Methodology: Quantitative Sandwich Immunoassay

Aviva's mouse Chemerin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Chemerin has been precoated onto 96-well plates. Standards(E.coli, T20-K162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chemerin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Chemerin amount of sample captured in plate.