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ADAMTS13 ELISA Kit (Human)

ADAMTS13 ELISA Kit (Human)

Item no.

OKBB00851

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Conjugate/Tag

Biotin

Sensitivity

<20 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

A disintegrin and metalloproteinase with thrombospondin motifs 13,A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13,A disintegrin like and metalloprotease with thrombospondin type 1 motif 13,A disintegrin-like and metalloprotease (Reprolysin type) with thrombospondin type 1 motif 13,ADAM metallopeptidase with thrombospondin type 1 motif 13,ADAM TS,ADAM-TS 13,ADAM-TS13,ADAMTS 13,ADAMTS-13,ADAMTS13,ADAMTS13 protein,ATS13_HUMAN,C9orf8,EC 3.4.24.-,TTP,Von Willebrand factor cleaving protease,Von Willebrand factor cleaving protease,von Willebrand factor-cleaving protease,vWF cleaving protease,vWF CP,vWF-cleaving protease,vWF-CP,vWFCP

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

ADAMTS13

Gene Fullname

ADAM metallopeptidase with thrombospondin type 1 motif, 13

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Nucleotide accession_num

NM_139025.4

Protein accession_num

NP_620594.1

Protein name

A disintegrin and metalloproteinase with thrombospondin motifs 13

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q34-W688

Manufacturer - Specificity

Natural and recombinant human ADAMTS13

Kit duration

~ 3 Hours

Kit principle

Aviva's human ADAMTS13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for ADAMTS13 has been precoated onto 96-well plates. Standards(CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ADAMTS13 amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma (heparin, EDTA, citrate)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB00851.pdf

OKBB00851-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 12/25/2025

Express Delivery: Save 1 days

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