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IL22 ELISA Kit (Human)

IL22 ELISA Kit (Human)

Item no.

OKBB00856

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Conjugate/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Cytokine Zcyto18,IL 21,IL 22,IL D110,IL-10-related T-cell-derived-inducible factor,IL-22,IL-TIF,Il22,IL22_HUMAN,ILTIF,Interleukin 22,Interleukin-22,TIFa,TIFIL 23,zcyto18

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

IL22

Gene Fullname

interleukin 22

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Interleukin-22(IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.

Nucleotide accession_num

NM_020525.4

Protein accession_num

NP_065386.1

Protein name

Interleukin-22

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A34-I179

Manufacturer - Specificity

Natural and recombinant human IL-22

Kit duration

~ 3 Hours

Kit principle

Aviva's human IL-22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-22 has been precoated onto 96-well plates. Standards(E.coli, A34-I179) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-22 amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB00856.pdf

OKBB00856-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 10/30/2025

Express Delivery: Save 1 days

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