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METRNL ELISA Kit (Mouse)

METRNL ELISA Kit (Mouse)

Item no.

OKBB01078

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Mouse (Murine, Mus musculus)

Conjugate/Tag

Biotin

Sensitivity

<12 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Meteorin-like protein,Metrnl,Subfatin,Q8VE43

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

METRNL

Gene Fullname

meteorin, glial cell differentiation regulator-like

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. In mice, exogenously elevated circulating Metrnl increased whole-body energy expenditure and improved glucose tolerance following diet-induced obesity. It also induced expression of antiinflammatory genes, leading to alternative activation of macrophages in adipose tissue and to browning of white fat depots. Metrnl did not appear to activate thermogenic genes directly, but it stimulated eosinophils and macrophages to enter adipose tissue and take their specific thermogenic actions. Blockade of cytokine signaling eliminated Metrnl-induced changes in gene expression required for alternative macrophage activation and blunted expression of beige fat thermogenic and beta-oxidation genes. It is concluded that METRNL is a hormone that influences energy expenditure and thermogenic programs in adipose tissue.

Nucleotide accession_num

NM_144797.3

Protein accession_num

NP_659046.1

Protein name

Meteorin-like protein

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q46-E311

Manufacturer - Specificity

Natural and recombinant mouse Meteorin-like

Kit duration

~ 3 Hours

Kit principle

Aviva's mouse Meteorin-like ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Meteorin-like has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: Q46-E311) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Meteorin-like is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Meteorin-like amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB01078.pdf

OKBB01078-datasheet.pdf

Request safety datasheet

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 12/25/2025

Express Delivery: Save 3 days

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