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Efemp1 ELISA Kit (Mouse)

Efemp1 ELISA Kit (Mouse)

Item no.

OKBB01113

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Mouse (Murine, Mus musculus)

Conjugate/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

EGF-containing fibulin-like extracellular matrix protein 1; Fibulin-3; FIBL-3; Efemp1; Fbln3

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

EFEMP1

Gene Fullname

epidermal growth factor-containing fibulin-like extracellular matrix protein 1

Reconstitution and storage

Store at 4C for 6 months, at -20C for 15 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.

Nucleotide accession_num

NM_146015.2

Protein accession_num

NP_666127.2

Protein name

EGF-containing fibulin-like extracellular matrix protein 1

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q18-F493

Manufacturer - Specificity

Natural and recombinant mouse EFEMP1

Kit duration

~ 3 Hours

Kit principle

Aviva's mouse EFEMP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EFEMP1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: Q18-F493ï¼‰ and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EFEMP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse EFEMP1 amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB01113.pdf

OKBB01113-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 10/30/2025

Express Delivery: Save 1 days

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