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Mag ELISA Kit (Rat)

Mag ELISA Kit (Rat)

Item no.

OKBB01429

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Rat (Rattus norvegicus)

Conjugate/Tag

Biotin

Sensitivity

<50 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Myelin-associated glycoprotein; 1B236; Brain neuron cytoplasmic protein 3; Sialic acid-binding Ig-like lectin 4a; Siglec-4a; Mag

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

MAG

Gene Fullname

myelin-associated glycoprotein

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Myelin-associated glycoprotein (MAG, Siglec-4) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NoGo-66 receptor(NgR). This gene is mapped to 1q21. The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.

Nucleotide accession_num

NM_017190.4

Protein accession_num

NP_058886.1

Protein name

Myelin-associated glycoprotein

Immunogen

Expression system for standard: NS0; Immunogen sequence: G20-P516

Manufacturer - Specificity

Natural and recombinant rat MAG

Kit duration

~ 3 Hours

Kit principle

Aviva's rat MAG ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MAG has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: G20-P516) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MAG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat MAG amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma (heparin, EDTA, citrate)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB01429.pdf

OKBB01429-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 12/25/2025

Express Delivery: Save 2 days

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