CA50 ELISA Kit (Human)

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CA50

This assay has high sensitivity and excellent specificity for detection of human
CA50. No significant cross-reactivity or interference between human CA50 and
analogues was observed.

2.5 Hours

Aviva Systems Biology CA50 ELISA Kit (Human) (OKCA00106) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A mouse monoclonal antibody specific for human CA50 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for human CA50 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human CA50 captured in well.