CXCL12 ELISA Kit (Human)

ELISA Kit

Wet Ice

C-X-C motif chemokine ligand 12

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.

NP_000600.1

Assay Methodology: Quantitative Sandwich ELISA

Dilution - Mean n=4 (%) - Range (%)
1:5 - 88 - 85-92
1:10 - 96 - 91-102
1:20 - 92 - 86-98
1:40 - 90 - 85-96

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 93 - 87-96
EDTA Plasma (n=4) - 95 - 90-99

Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates