HTR2A ELISA Kit (Rat)

ELISA Kit

Wet Ice

5-hydroxytryptamine receptor 2A

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2, 5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.

NP_058950.1

Assay Methodology: Quantitative Sandwich ELISA

Dilution - Mean n=4 (%) - Range (%)
Neat - 87 - 82-91
1:2 - 104 - 98-110
1:4 - 90 - 85-94
1:8 - 84 - 80-88

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 89 - 84-96
EDTA Plasma (n=4) - 95 - 90-103

Serum, Plasma, Tissue Homogenates, Cell Lysates