Comparison

CD14 ELISA Kit (Rat)

Item no. OKCA01002
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Specific against Rat (Rattus norvegicus)
Sensitivity 7 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
CD14
Gene Fullname
Monocyte differentiation antigen CD14
Reconstitution and storage
Store as indicated in product manual.
Description of target
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL-.
Nucleotide accession_num
NM_001082195.2
Protein accession_num
NP_001075664.1
Protein name
Monocyte differentiation antigen CD14
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit duration
~ 3.5 Hours
Kit linearity
Dilution - Mean n=4 (%) - Range (%)
Neat - 94 - 86-100
1:2 - 99 - 90-103
1:4 - 89 - 83-92
1:8 - 95 - 84-98
Kit principle
Aviva Systems Biology CD14 ELISA Kit (Rat) (OKCA01002) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CD14 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD14 captured in well.
Kit recovery

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 102 - 94-108
EDTA Plasma (n=4) - 96 - 89-101
Kit reproducibility
Intra-assay Precision: 3 samples with known low, middle and high level CD14 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CD14 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Sample Type
Serum, Plasma, Tissue Homogenates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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