TJP1 ELISA Kit (Rat)

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TJP1

Store as indicated in product manual.

NM_001003140.1

Tight junction protein ZO-1

~ 3.5 Hours

Aviva Systems Biology TJP1 ELISA Kit (Rat) (OKCA01006) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for TJP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TJP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TJP1 captured in well.

Intra-assay Precision: 3 samples with known low, middle and high level TJP1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level TJP1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%