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TMEM119 ELISA Kit (Human)

TMEM119 ELISA Kit (Human)

Item no.	OKCA01559
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Neural Development Cell Biology Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Sensitivity	15.6 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	OBIF
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	TMEM119
Gene Fullname	transmembrane protein 119
Reconstitution and storage	Store as indicated in product manual.
Description of target	Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts (PubMed:20025746). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.
Nucleotide accession_num	NM_181724.2
Protein accession_num	NP_859075.2
Protein name	Transmembrane protein 119
Assay info	Assay Methodology: Quantitative Sanadwich ELISA
Kit duration	~ 3.5 Hours
Kit linearity	Dilution - Mean Recovery (%) - Range (%) Neat - 107 - 103-110 \| 1:2 - 88 - 84-92 \| 1:4 - 107 - 103-110 \| 1:8 - 90 - 87-93
Kit principle	Aviva Systems Biology TMEM119 ELISA Kit (Human) (OKCA01559) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TMEM119 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TMEM119 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TMEM119 captured in the well.
Kit recovery	Sample - Mean Recovery (%) - Range (%) Serum (n=5) - 104 - 100-108 EDTA Plasma (n=4) - 88 - 84-93
Sample Type	Serum, Plasma, Tissue Homogenates, Cell Lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCA01559.pdf

OKCA01559-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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