MMP14 ELISA Kit (Rat)

ELISA Kit

Wet Ice

Matrix metalloproteinase-14

Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues.

NP_001076262.1

Assay Methodology: Quantitative Sanadwich ELISA

Aviva Systems Biology MMP14 ELISA Kit (Rat) (OKCA01903) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP14 captured in the well.

Serum, Plasma And Tissue Homogenates