SFTPC ELISA Kit (Rat)

ELISA Kit

Wet Ice

Pulmonary surfactant-associated protein C

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.

NP_001075791.1

Assay Methodology: Quantitative Sanadwich ELISA

Aviva Systems Biology SFTPC ELISA Kit (Rat) (OKCA01925) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPC has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SFTPC captured in the well.

Serum, Plasma, Tissue Homogenates