ICAM1 ELISA Kit (Dog)

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ICAM1

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

NP_001003291

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology ICAM1 ELISA Kit (Dog) (OKCA02020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ICAM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ICAM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ICAM1 captured in well.

Serum, Plasma, Tissue Homogenates