EFNA1 ELISA Kit (Mouse)

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EFNA1

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.

NP_034237

Assay Methodology: Quantitative Sandwich Immunoassay

Dilution - Mean Recovery (%) - Range (%)
Neat - 84 - 80-88
1:2 - 96 - 93-99
1:4 - 87 - 83-90
1:8 - 105 - 100-110

Sample Type - Mean Recovery (%) - Range (%)
Serum (n=5) - 87 - 80-94
EDTA Plasma (n=4) - 102 - 98-106

Serum, Plasma, Tissue Homogenates