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ITGAM ELISA Kit (Mouse)

ITGAM ELISA Kit (Mouse)

Item no.	OKCA02400
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neural Lineage Markers Aging & Neurological Disorders By Cell Type Hematology Myeloma Cell Biology Cell Movement Cell Adhesion ELISA ELISA & Immunoassays ELISA Kits Immunoassay Oligodendrocyte Markers
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	3.9 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD11B,CR3A,MAC-1,MAC1A,MGC117044,MO1A,SLEB6,antigen CD11b (p170),integrin alpha M,macrophage antigen alpha polypeptide,neutrophil adherence receptor alpha-M subunit
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	ITGAM
Reconstitution and storage	Store as indicated in product manual.
Description of target	Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.
Protein name	Integrin alpha-M
Assay info	Assay Methodology: Quantitative Sandwich Immunoassay
Kit duration	~ 3.5 Hours
Kit linearity	Dilution - Mean Recovery (%) - Range (%) 1:100 - 86 - 81-90 1:200 - 103 - 99-108 1:400 - 97 - 92-101 1:800 - 90 - 86-94
Kit principle	Aviva Systems Biology Itgam ELISA Kit (Mouse) (OKCA02400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Itgam has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Itgam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Itgam captured in the well.
Kit recovery	Sample Type - Mean Recovery (%) - Range (%) Serum (n=5) - 95 - 90-100 EDTA Plasma (n=4) - 88 - 84-92
Kit reproducibility	Mean Intra-Assay: CV <12% (n=20) Mean Inter-Assay: CV <15% (n=24)
Sample Type	Serum, Plasma, Cell Culture Supernates, Tissue Homogenates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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