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CXCR4 ELISA Kit (Rat)

CXCR4 ELISA Kit (Rat)

Item no.	OKCA02541
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Molecular Targets for Neuroscience Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.039 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD184,D2S201E,FB22,HM89,HSY3RR,LAP3,LCR1,LESTR,NPY3R,NPYR,NPYRL,NPYY3R,WHIM,C-X-C chemokine receptor type 4,CD184 antigen,fusin,leukocyte-derived seven-transmembrane-domain receptor,lip
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CXCR4
Reconstitution and storage	Store as indicated in product manual.
Description of target	Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Protein name	C-X-C chemokine receptor type 4
Assay info	Assay Methodology: Quantitative Sandwich Immunoassay
Kit duration	~ 3.5 Hours
Kit linearity	Dilution - Mean Recovery (%) - Range (%) Neat - 92 - 86-95 1:2 - 103 - 97-106 1:4 - 93 - 85-96 1:8 - 97 - 91-100
Kit principle	Aviva Systems Biology Cxcr4 ELISA Kit (Rat) (OKCA02541) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcr4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cxcr4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cxcr4 captured in the well.
Kit recovery	Sample Type - Mean Recovery (%) - Range (%) Serum (n=5) - 95 - 89-98 EDTA Plasma (n=4) - 96 - 92-99
Kit reproducibility	Mean Intra-Assay: CV <12% (n=20) Mean Inter-Assay: CV <15% (n=24)
Sample Type	Serum, Plasma, Cell Culture Supernates, Tissue Homogenates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCA02541.pdf

OKCA02541-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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