TP53 ELISA Kit (Rat)

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TP53

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.

NP_001075873

Assay Methodology: Quantitative Sandwich Immunoassay

Dilution - Mean Recovery (%) - Range (%)
Neat - 100 - 95-108
1:2 - 87 - 82-91
1:4 - 90 - 85-94
1:8 - 92 - 88-96

Sample Type - Mean Recovery (%) - Range (%)
Serum (n=5) - 89 - 84-93
EDTA Plasma (n=4) - 95 - 90-100

Serum, Plasma, Tissue Homogenates