STIP1 ELISA Kit (Human)

ELISA Kit

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STIP1

543

Acts as a co-chaperone for HSP90AA1 (PubMed:27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).

NP_001269581

This assay has high sensitivity and excellent specificity for detection of Stress Induced Phosphoprotein 1 (STIP1).No significant cross-reactivity or interference between Stress Induced Phosphoprotein 1 (STIP1) and analogues was observed.

Component: Amount
Anti-STIP1 Microplate: 96 Wells (12 x 8 Well Strips)
STIP1 Lyophilized Standard: 2 x 100 ng
100X Biotinylated STIP1 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stress Induced Phosphoprotein 1 (STIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stress Induced Phosphoprotein 1 (STIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stress Induced Phosphoprotein 1 (STIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Stress Induced Phosphoprotein 1 (STIP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stress Induced Phosphoprotein 1 (STIP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stress Induced Phosphoprotein 1 (STIP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%