TIGIT ELISA Kit (Human)

ELISA Kit

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TIGIT

244

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

NP_776160

This assay has high sensitivity and excellent specificity for detection of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT).No significant cross-reactivity or interference between T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) and analogues was observed.

Component: Amount
Anti-TIGIT Microplate: 96 Wells (12 x 8 Well Strips)
TIGIT Lyophilized Standard: 2 x 20 ng
100X Biotinylated TIGIT Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%