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Nptx1 ELISA Kit (Rat)

Nptx1 ELISA Kit (Rat)

Item no.	OKCD01505
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Metabolism Glucose Homeostasis Cell Biology Cell Status Necrosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	< 5.5 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	47 kDa taipoxin-binding protein;neuronal pentraxin I;neuronal pentraxin-1;NP1;NP-I.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Nptx1
Gene Fullname	neuronal pentraxin 1
Protein size	432
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
Nucleotide accession_num	NM_153735.2
Protein accession_num	NP_714957.1
Protein name	Neuronal pentraxin-1
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Neuronal Pentraxin I (NPTX1).No significant cross-reactivity or interference between Neuronal Pentraxin I (NPTX1) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components	Component: Amount Anti-Nptx1 Microplate: 96 Wells (12 x 8 Well Strips) Nptx1 Lyophilized Standard: 2 x 1 ng 100X Biotinylated Nptx1 Detector Antibody: 120 uL Avidin/HRP Conjugate: 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 9 mL Stop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal Pentraxin I (NPTX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuronal Pentraxin I (NPTX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuronal Pentraxin I (NPTX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuronal Pentraxin I (NPTX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuronal Pentraxin I (NPTX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuronal Pentraxin I (NPTX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD01505.pdf

OKCD01505-datasheet.pdf

OKCD01505-safety-datasheet.pdf