Comparison

GDF6 ELISA Kit (Human)

Item no. OKCD01632
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity < 0.059 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias BMP13;BMP-13;bone morphogenetic protein 13;CDMP2;growth/differentiation factor 16;growth/differentiation factor 6;KFM;KFS;KFS1;KFSL;SGM1;SYNS4.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
GDF6
Gene Fullname
growth differentiation factor 6
Protein size
455
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Growth factor that controls proliferation and cellular differentiation in the retina and bone formation. Plays a key role in regulating apoptosis during retinal development. Establishes dorsal-ventral positional information in the retina and controls the formation of the retinotectal map (PubMed:23307924). Required for normal formation of bones and joints in the limbs, skull, digits and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Regulation of GDF6 expression seems to be a mechanism for evolving species-specific changes in skeletal strucutres. Seems to positively regulates differentiation of chondrogenic tissue through the growth factor receptors subunits BMPR1A, BMPR1B, BMPR2 and ACVR2A, leading to the activation of SMAD1-SMAD5-SMAD8 complex. The regulation of chondrogenic differentiation is inhibited by NOG (PubMed:26643732). Also involved in the induction of adipogenesis from mesenchymal stem cells. This mechanism acts through the growth factor receptors subunits BMPR1A, BMPR2 and ACVR2A and the activation of SMAD1-SMAD5-SMAD8 complex and MAPK14/p38 (By similarity).
Nucleotide accession_num
NM_001001557.2
Protein accession_num
NP_001001557.1
Protein name
Growth/differentiation factor 6
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 6 (GDF6).No significant cross-reactivity or interference between Growth Differentiation Factor 6 (GDF6) and analogues was observed.
Assay info
Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components
Component: Amount
Anti-GDF6 Microplate: 96 Wells (12 x 8 Well Strips)
GDF6 Lyophilized Standard: 2 x 10 ng
100X Biotinylated GDF6 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 95-102% - 97-105% - 80-105% - 90-102%
EDTA Plasma (n=5) - 79-104% - 90-103% - 84-93% - 95-102%
Heparin Plasma (n=5) - 81-90% - 80-96% - 80-101% - 84-99%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 6 (GDF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 6 (GDF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Differentiation Factor 6 (GDF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Growth Differentiation Factor 6 (GDF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery Range (%) - Average (%)
Serum (n=5) - 97-105 - 101
EDTA Plasma (n=5) - 79-104 - 91
Heparin Plasma (n=5) - 78-104 - 93
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 6 (GDF6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 6 (GDF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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