Comparison

Rspo2 ELISA Kit (Mouse)

Item no. OKCD02065
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity < 29 pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias 2610028F08Rik;AA673245;cristin-2;cysteine-rich and single thrombospondin domain-containing protein 2;D430027K22;ft;ftls;mCristin-2;roof plate-specific spondin-2;R-spondin 2 homolog;R-spondin-2.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Rspo2
Gene Fullname
R-spondin 2
Protein size
243
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors.
Nucleotide accession_num
NM_172815.3
Protein accession_num
NP_766403.1
Protein name
R-spondin-2
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of R-Spondin 2 (RSPO2).No significant cross-reactivity or interference between R-Spondin 2 (RSPO2) and analogues was observed.
Assay info
Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components
Component: Amount
Anti-Rspo2 Microplate: 96 Wells (12 x 8 Well Strips)
Rspo2 Lyophilized Standard: 2 x 5 ng
100X Biotinylated Rspo2 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 84-95% - 82-97% - 87-103% - 81-97%
EDTA Plasma (n=5) - 78-99% - 94-105% - 91-101% - 81-88%
Heparin Plasma (n=5) - 88-96% - 79-91% - 96-105% - 92-101%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 2 (RSPO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 2 (RSPO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 2 (RSPO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of R-Spondin 2 (RSPO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery Range (%) - Average (%)
Serum (n=5) - 88-95 - 91
EDTA Plasma (n=5) - 88-95 - 91
Heparin Plasma (n=5) - 95-103 - 99
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close