C-Peptide ELISA Kit (Dog)

ELISA Kit

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INS

2°C to 8°C|-20°C

Insulin

Component: Amount
Anti-C-Peptide Microplate: 96 Wells (12 x 8 Well Strips)
C-Peptide Lyophilized Standard: 2
C-Peptide Biotin Complex: 1
100X Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Biotin Complex Diluent: 1 x 12 mL
Avidin/HRP Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL
Biotin Complex Reconstitution Buffer: 1 x 300 uL

2h

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to C-Peptide (CP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled C-Peptide (CP) and unlabeled C-Peptide (CP) (Standards or samples) with the pre-coated antibody specific to C-Peptide (CP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of C-Peptide (CP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of C-Peptide (CP) in the sample.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Peptide (CP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Peptide (CP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%