Leukotriene B4 ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

2°C to 8°C|-20°C

Leukotriene B4

Component: Amount
Anti-Leukotriene B4 Microplate: 96 Wells (12 x 8 Well Strips)
Leukotriene B4 Lyophilized Standard: 2
100X Leukotriene B4 Biotin Complex: 120 uL
100X Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Biotin Complex Diluent: 1 x 12 mL
Avidin/HRP Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

2h

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene B4 (LTB4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene B4 (LTB4) and unlabeled Leukotriene B4 (LTB4) (Standards or samples) with the pre-coated antibody specific to Leukotriene B4 (LTB4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene B4 (LTB4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene B4 (LTB4) in the sample.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukotriene B4 (LTB4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukotriene B4 (LTB4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%