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ADAM17 ELISA Kit (Rat)

ADAM17 ELISA Kit (Rat)

Item no.	OKCD02296
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Molecular Targets for Neuroscience Infectious Diseases Viral Infections Coronavirus (COVID-19) By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA Cardiovascular Research Heart Failure Myocardial Infarction ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.061 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	a disintegrin and metallopeptidase domain 17;a disintegrin and metalloprotease domain 17;a disintegrin and metalloproteinase domain 17 (tumor necrosis factor,alpha,converting enzyme);ADAM 17;disintegrin and metalloproteinase domain-containing protein 17;TACE;TNF-alpha convertase;TNF-alpha-converting enzyme.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit, Root Catalog/Research Areas/Neuroscience ELISA Kits
Shipping Temperature	Wet Ice
Gene symbol	Adam17
Gene Fullname	ADAM metallopeptidase domain 17
Protein size	827
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2 (By similarity).
Nucleotide accession_num	NM_020306.2
Protein accession_num	NP_064702.1
Protein name	Disintegrin and metalloproteinase domain-containing protein 17
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 17 (ADAM17).No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 17 (ADAM17) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Anti-ADAM17 Microplate: 96 Wells (12 x 8 Well strips) ADAM17 Lyophilized Standard: 2 100X Biotinylated ADAM17 Detector Antibody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL Stop Solution: 1 x 6 mL TMB Substrate: 1 x 9 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 17 (ADAM17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of A Disintegrin And Metalloprotease 17 (ADAM17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 17 (ADAM17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD02296.pdf

OKCD02296-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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