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AQP5 ELISA Kit (Rat)

AQP5 ELISA Kit (Rat)

Item no.	OKCD02310
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Molecular Targets for Neuroscience ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.125 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	AQP-5;aquaporin-5.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Aqp5
Gene Fullname	aquaporin 5
Protein size	265
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells.
Nucleotide accession_num	NM_012779.1
Protein accession_num	NP_036911.1
Protein name	Aquaporin-5
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Aquaporin 5 (AQP5).No significant cross-reactivity or interference between Aquaporin 5 (AQP5) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Anti-AQP5 Microplate: 96 Wells (12 x 8 Well strips) AQP5 Lyophilized Standard: 2 100X Biotinylated AQP5 Detector Antibody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL Stop Solution: 1 x 6 mL TMB Substrate: 1 x 9 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 5 (AQP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 5 (AQP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 5 (AQP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aquaporin 5 (AQP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 5 (AQP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 5 (AQP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD02310.pdf

OKCD02310-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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