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EGR1 ELISA Kit (Mouse)

EGR1 ELISA Kit (Mouse)

Item no.	OKCD02519
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma Mesothelioma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Astrocytoma By Organ Colorectal Cancer Breast Cancer Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	0.058 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	A530045N19Rik;early growth response protein 1;egr;Egr-;Egr-1;ETR10;ETR103;Krox;Krox-;Krox-1;Krox24;Krox-24;Krox-24 nuclear protein;nerve growth factor-induced protein A;NG;NGF;NGF1;NGF1-A;NGFIA;NGFI-A;TI;TIS8;transcription factor Zif268;Zen;Zenk;Zfp-;Zfp-6;Zif26;Zif268;zinc finger protein Krox-24.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Egr1
Gene Fullname	early growth response 1
Protein size	533
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Transcriptional regulator (PubMed:8336701, PubMed:8703054, PubMed:15958557). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (PubMed:8703054, PubMed:15958557, PubMed:2028256, PubMed:8939742). Binds double-stranded target DNA, irrespective of the cytosine methylation status (By similarity). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia (PubMed:11100120, PubMed:15958557). Plays a role in the regulation of cell survival, proliferation and cell death (PubMed:15265859, PubMed:15958557). Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation (PubMed:15958557). Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy (PubMed:15265859). Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia (PubMed:11100120). Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (PubMed:8703054).
Nucleotide accession_num	NM_007913.5
Protein accession_num	NP_031939.1
Protein name	Early growth response protein 1
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Early Growth Response Protein 1 (EGR1).No significant cross-reactivity or interference between Early Growth Response Protein 1 (EGR1) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Anti-EGR1 Microplate: 96 Wells (12 x 8 Well strips) EGR1 Lyophilized Standard: 2 100X Biotinylated EGR1 Detector Antibody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL Stop Solution: 1 x 6 mL TMB Substrate: 1 x 9 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Early Growth Response Protein 1 (EGR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Early Growth Response Protein 1 (EGR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Early Growth Response Protein 1 (EGR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Early Growth Response Protein 1 (EGR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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