HYAL1 ELISA Kit (Rat)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

hyaluronidase 1

2°C to 8°C|-20°C

NM_207616.1

Hyaluronidase-1

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronoglucosaminidase 1 (HYAL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronoglucosaminidase 1 (HYAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronoglucosaminidase 1 (HYAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hyaluronoglucosaminidase 1 (HYAL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tissue homogenates and other biological fluids