IDE ELISA Kit (Rat)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

insulin degrading enzyme

2°C to 8°C|-20°C

NM_013159.1

Insulin-degrading enzyme

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Degrading Enzyme (IDE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Degrading Enzyme (IDE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Degrading Enzyme (IDE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Degrading Enzyme (IDE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tissue homogenates, cell lysates and other biological fluids