« Back
Home /
PDX1 ELISA Kit (Mouse)

PDX1 ELISA Kit (Mouse)

Item no.	OKCD02650
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Metabolism Diabetes Glucose Homeostasis ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	0.054 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IDX-;IDX-1;Insulin promoter factor 1;insulin promoter factor 1,homeodomain transcription factor;Ip;IPF;Ipf1;IPF-1;islet/duodenum homeobox 1;Mody;Mody4;pancreas/duodenum homeobox protein 1;pancreatic and duodenal homeobox gene 1;pdx-;pdx-1;somatostatin-transactivating factor 1;STF-;STF-1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Pdx1
Gene Fullname	pancreatic and duodenal homeobox 1
Protein size	284
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.
Nucleotide accession_num	NM_008814.3
Protein accession_num	NP_032840.1
Protein name	Pancreas/duodenum homeobox protein 1
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Insulin Promoter Factor 1 (IPF).No significant cross-reactivity or interference between Insulin Promoter Factor 1 (IPF) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Anti-PDX1 Microplate: 96 Wells (12 x 8 Well strips) PDX1 Lyophilized Standard: 2 100X Biotinylated PDX1 Detector Antibody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL Stop Solution: 1 x 6 mL TMB Substrate: 1 x 9 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Promoter Factor 1 (IPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Promoter Factor 1 (IPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Promoter Factor 1 (IPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Promoter Factor 1 (IPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Promoter Factor 1 (IPF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Promoter Factor 1 (IPF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD02650.pdf

OKCD02650-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 96 Wells

available

Delivery expected until 12/25/2025

Express Delivery: Save 2 days

Compare

Add to wishlist

Get an offer