NEFH ELISA Kit (Rat)

ELISA Kit

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NEFH

1072

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

NP_036739.2

This assay has high sensitivity and excellent specificity for detection of Neurofilament, Heavy Polypeptide (NEFH).No significant cross-reactivity or interference between Neurofilament, Heavy Polypeptide (NEFH) and analogues was observed.

Component: Amount
Anti-NEFH Microplate: 96 Wells (12 x 8 Well strips)
NEFH Lyophilized Standard: 2
100X Biotinylated NEFH Detector Antibody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
Stop Solution: 1 x 6 mL
TMB Substrate: 1 x 9 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Heavy Polypeptide (NEFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neurofilament, Heavy Polypeptide (NEFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurofilament, Heavy Polypeptide (NEFH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurofilament, Heavy Polypeptide (NEFH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%